Abstract
A method for the determination of sucralose in various foods by RI-HPLC and ion chromatography with a pulsed amperometric detector (PAD-IC) was developed.
Chopped or homogenized samples were packed into cellulose tubing with 0.01 mol/L hydrochloric acid containing 10% sodium chloride and were dialyzed against 0.01 mol/L hydrochloric acid for 24 hours. The dialyzate was passed through a Bond Elut ENV cartridge, and the cartridge was washed with 0.2 mol/L NaOH and water. Sucralose was eluted from the cartridge with methanol. The extract was taken to dryness in an evaporator and the residue was re-dissolved in water.
Sucralose was separated on an Inertsil ODS-3V column with a mobile phase of acetonitrile-water (15 : 85) and an RI detector. It was also determined on a CarboPak PA1 column with a mobile phase of 100 mmol/L NaOH-75 mmol/L CH3COONa, using a PAD detector.
The recoveries of sucralose from various kinds of foods spiked at 50 μg/g and 200 μg/g ranged from 88∼105%. The detection limit in samples was 10 μg/g for RI-HPLC and 1 μg/g for PAD-IC.
