Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
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Study on Positive Control for GM Papaya (55-1) Detection Method by GUS (β-Glucuronidase) Assay
Kunihiko TAKAHASHIMasakazu HORIE
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2005 Volume 46 Issue 2 Pages 55-57

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Abstract
A suitable positive control was investigated for histochemical assay (GUS-examining method) to detect genetically modified (GM) papaya (55-1), currently undergoing a safety assessment in Japan. Six different kinds of test papers were soaked with β-glucuronidase solution and examined for GUS activity. The test papers made of nylon and glass fiber turned blue, and were stable for fifteen months at −20°C. They are concluded to be useful as positive controls in the GUS-examining method for inspection of GM papaya (55-1).
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© 2005 Japanese Society for Food Hygiene and Safety
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