Inter-laboratory Validation Studies of Analytical Method for Determination of Chloramphenicol in Royal Jelly

Abstract

Inter-laboratory validation studies were conducted in 6 laboratories to validate the analytical method for determination of chloramphenicol in royal jelly. Chloramphenicol spiked at the levels of 0.1 and 0.5 ppm was analyzed. Mean recoveries were 89 and 89%, reproducibility relative standard deviations (RSD_R) were 10.5 and 6.8%, HORRAT_R values were 0.5 and 0.4. Samples containing residues at the levels of 0.25 and 0.80 ppm were analyzed. Mean recoveries were 89 and 84%, RSD_R were 9.8 and 12.3%, and HORRAT_R values were 0.5 and 0.7. The determination limit was 0.05 ppm. These results show that this method has good performance.