Abstract
The low molecular hydrolyzates obtained under hydrolysis of sodium carboxymethyl-starch (Na-CMS) with human saliva amylase was hydrolyzed again with some carboxylases or hydrochloric acid. The substances were almost unhydrolyzed with carboxylases, however, was hydrolyzed with hydrochloric acid.
Paper chromatogram of the hydrolyzates showed apparant four spots, and these were the same Rf values as glucose, maltose, maltotriose and maltotetraose. On each of these hydrolyzates purified with DEAE ion exchange cellulose the carboxymethyl group was recognized by the absorption spectra of the color produced with 2, 7-dihydroxynaphthalene in sulfuric acid and infrared spectra, and the degree of substitution were between 0.71 and 1.03.
