Studies on the Identification of Amino Acids by Paper Electrophoresis

Abstract

The identification of 19 protein composing amino acids by paper electrophoretic method was investigated.
By using 5N acetic acid as the electrolyte, 19 amino acids were grouped into four groups, that is A (Lys, Arg, His, Gly, Ala), B (Val, Leu, Ileu, Ser, Thr), C (Ser, Thr, Met, Glu, Pro, Phe), D (Tyr, Cys, Asp, Try, Hypro), and the individual amino acid in these groups was identified by the further electrophoresis with 3% aqueous ammonia as the electrolyte.
Amino acids of E group (Thr, Met, Phe) and F group (Tyr, Hypro), could not be separated in this case, were tried with 1% borax as the electrolyte.
By the above mentioned method, the amino acids, in this study, could be separated and identified mutually, except the discrimination between Leu and Ileu, Met and Phe.