Abstract
Sodium cyclamate, which had been forbidden to use as food additive to any kinds of foods, was separated from other food components by ultrafiltration and determined by colorimeteric method.
It has been reported that sodium cyclamate reacts with nitrite and turns into cyclohexyl nitrite.
In this report, the experimental condition for the colorimetric determination of the cyclohexyl nitrite was examined. Sulfanilamide and N-1-naphthylethylenediamine dihydrochloride were used as the reagents. The colored solution was determined at about 555mμ. of wave length Ultrafiltration method was found to be very effective for preparing the test solution, and the prepared test solution could be used for esterification directly without dilution, the solution not being emulsified with organic solvent on extraction procedure.
In the recovery test of cyclamate on food, the recovery ratio was about 90%.
The minimum limit concentrations of detectable sodium cyclamate were 50ppm in juice and was 500ppm in the other foods.
