Volume 13 (1972) Issue 5 Pages 368-375
Using the procedure employed for the separation of fusarenon and nivalenol, toxic substances were separated from cereal grains infected with Fusarium in the crop field of Kagawa-ken in 1970. Production of the toxic substances in culture filtrates of the isolated strain was also determined. The results are summarized as follows:
(1) Fusarium spp., Alternaria spp., Aspergillus spp. and Penicillium spp. were isolated from moldy grains of barley and wheat. Fusarium roseum or related species were found to be a majority of casual fungi.
(2) Moldy grains mainly contained “Rd-toxin”, mp. 149. 5-151°C, and nivalenol, mp. 224-226°C, both toxic to mice and protozoa and the former was considered as a newly found mycotoxin having a scirpene nucleus.
(3) Phytotoxic butenolide, mp. 115-117°C, hardly detectable in the grains, and Rd-toxin were separated from culture filtrates of the isolated Fusarium roseum strain, whereas nivalenol was not detected.