Abstract
An attempt was made to determine of penicillic acid and patulin simultaneously in brown rice, highly milled rice, wheat flour, milled barley and soy bean, applying thin layer chromatography (TLC) and gas liquid chromatography (GLC).
Twenty five grams of each sample was mixed with 100ml of methanol-5% saline solution (1:1) plus 75ml of n-hexane and shaken on a mechanical shaker for 30min and then 50ml of methanol-5% saline solution was separated through filter paper.
The separated solution, after being washed with 30ml of n-hexane and evaporated methanol, was re-extracted twice with 25ml of ethyl acetate.
Ethyl acetate extract was treated by silica gel column chromatography using a mixture of chloroform-methanol (97:3) as a developing solvent and then submitted to the determination with TLC and GLC.
TLC was performed applying silica gel plate and a mixture of chloroform-methanol (93:7) as a developing solvent, and 1% ammonia water and 4% phenylhydrazine hydrochloride solution as a spray reagents.
GLC was performed applying a flame ionization detector and mixed column of 15% QF-1+10% DC-200 (55:45) after treated with TMS-reagents.
The limits of detection of penicillic acid and patulin in cereal were about 0.2ppm. Recovery rates of penicillic acid in cereal were 74.6-87.6% and that of patulin were 72.9-79.5%.
Furthermore, this simultaneous analysis was demonstrated in moldy rice.
