Abstract
Quantitative analysis of sorbic acid permitted as a food additive was completed by gas chromatography.
Both of sorbic acid methylated with diazomethane and anisole, the internal standard substance, were diluted to 10ml with ethyl acetate.
The calibration curves were obtained by plotting the relative peak heights according to the content of sorbic acid through polyvinyl acetate column and silicone grease column.
When Ps/Pa was a value from only one experiment, the error of calibration curve was larger than the mean value of results from three experiments, the error of which was not more than 3% in the case of more than 50mg Sorbic acid in 10ml.
In the case of determining sorbic acid in food by gas chromatography with the thermal conductivity cell, we need a large amount of sample (about 100g). But as the previously reported methods (the distillation method) were not efficient in extracting the acid from a large quantity of food, it is necessary to study a new measure for recovering the acid from foods.
