Methods for the Detection of Residual Hygromycin B in Animal Tissues

Abstract

Experimental studies on the detection of residual hygromycin B in animal tissues were carried out by bioautography on thin-layer chromatographic plates, with Bacillus subtilis ATCC 6633.
A linear calibration plot was obtained in the range of 0.0625 to 1.0 unit per spot of hygromycin B standard solution by bioautography.
The assay sensitivity was improved to give a detection limit of 0.5 unit per spot (0.8 unit per gram) in muscles and liver and 1.0 unit per spot (1.6 unit per gram) in kidneys of pigs.
The average recoveries in the muscles, liver and kidneys were 57.1%, 50.9% and 22.0%, respectively.