Abstract
A high performance liquid chromatographic (HPLC) method was established for the quantitative determination of sesamolin, sesamin and sesamol in sesame oil.
Sesamolin, sesamin and sesamol were eluted at approximately 8min, 13min and 35min by using a 4.6mm∅×250mm Lichrosorb-Si 60 column with n-hexane·chloroform (80:20). The ultraviolet absorption detector was set at 280nm. Sesame oil was diluted with the eluting solvent and a sample was directly injected into the HPLC machine.
The results were as follows:
1. The extracted sesame oil were found to contain 0.42% sesamolin and 0.54% sesamin.
2. No sesamol was detected in a survey of sesame oil by this procedure (detection limit 0.0005%).
3. The average recoveries of sesamolin, sesamin and sesamol from sesame oil were 99.3%, 97.3%, 99.0%, respectively.
