1985 Volume 26 Issue 6 Pages 585-590_1
A sensitive and simple method for determination of monensin in chicken tissues by high performance liquid chromatography (HPLC) with a fluorescence detector was developed.
Monensin was extracted with a mixture of 0.5% metaphosphoric acid and methanol (1:1) from samples and reextracted with chloroform. The chloroform extract was evaporated to dryness. Monensin in the residue was converted into the corresponding ADAM-monensin by reaction with 9-anthryldiazomethane (ADAM). After clean-up with a Sep-pak silica cartridge, ADAM-monensin derivative was determined by HPLC with a fluorescence detector (excitation at 365nm, emission at 412nm).
The calibration graph was rectilinear from 0.1μg/ml to 1.0μg/ml. The average recoveries of monensin from chicken meat and liver fortified at the level of 0.5ppm were 82.1% for meat and 67.8% for liver.
