Determination of Bitertanol in Banana by Liquid Chromatography

Abstract

A simple method for determination of bitertanol in banana by high performance liquid chromatography with fluorescence detection was developed. The banana sample homogenate was extracted with acetone. The crude extract was dissolved in 10% sodium chloride solution and ethyl acetate, then the ethyl acetate layer was concentrated to dryness. The residue was dissolved in n-hexane, and this solution was cleaned up with a Florisil^® Cartridge. A methanol solution was analyzed by liquid chromatography. Bitertanol was detected at 325nm (excitation at 265nm). Recoveries at the 0.1ppm and 0.5ppm levels were 93% with a coefficient of variation of 3.3%, and the detection limit was 0.005ppm in the samples.