The Journal of Silk Science and Technology of Japan
Online ISSN : 1881-1698
Print ISSN : 1880-8204
ISSN-L : 1880-8204
Production and Cell Adhesion Activity of Recombinant Full-length Hornet Silk Protein Fused with RGDS Peptide
Yusuke KambeTsunenori Kameda
Author information
JOURNALS FREE ACCESS

2014 Volume 22 Pages 47-49

Details
Abstract

Vssilk1, a major component of hornet silk produced by larvae of the yellow hornet (Vespa simillima), can be produced as a recombinant protein in Escherichia coli. Here, we successfully fused a tandem repeat of a cell adhesion peptide, Arg-Gly-Asp-Ser (RGDS), to full-length Vssilk1 protein by genetic recombination. The recombinant Vssilk1 protein with the RGDS sequence (Vssilk1-RGDS) was fabricated into a cast film. We observed that more fibroblasts adhered to, and spread out on, the Vssilk1-RGDS film than on a Vssilk1 film.

Information related to the author
© 2014 by The Japanese Society of Silk Science and Technology
Previous article Next article
feedback
Top