Characterization of Antheraea pernyi fibroin films from the aqueous solution prepared by an improved process

抄録

The Antheraea pernyi silk fibroin (A. pernyi fibroin) has been studied as an expecting material for medical use with good cell proliferation in tissue engineering thanks to its RGD sequence in its molecule that improves cell adhesion. We developed an improved process to make the aqueous solution stably without gelation during the dialysis process and reported it in our previous paper. In this study, we reported the characterization of our A. pernyi fibroin film fabricated from the aqueous solution prepared by the new process, and we compared the results with previously reported A. pernyi fibroin films. The secondary structure of A. pernyi fibroin was determined by ATR-FTIR measurement of the film, and the surface morphology was observed by AFM. The physicochemical properties of the surface were measured by contact angle and zeta potential. In order to evaluate the cell compatibility, cell adhesion and proliferation test, and cell mobility measurement were performed. The characteristic properties and cell behaviors on our A. pernyi fibroin film were then reported.