A simple method for the detection of protein digestion using a horizontal agarose gel elctrophoresis

Abstract

The aim of the present experiment is to help children understand function of proteins. For this purpose we chose pepsin assayed under various conditions. To measure the enzyme activity we applied a simple (and less expensive) method consisting of BSA (Bovine Serum Albumin) combined with BPB (Bromophenol Blue) as a substrate and an electrophoresis using horizontal agarose gels for the detection of protein digestion. Activity of the enzyme was rapidly and easily measured by the disappearance of the protein bands without staining the gels. Using this simple method, students can easily perform their own experiments designed by themselves. They will have enough time to discuss the results to understand the function of the enzyme. In this report we present some data of protein digestion obtained by this method.