Pump-probe TR-WAXS Distinguishes Activation from Heating Effects in Ultrafast Rhodopsin Dynamics

Konstantinos Karpos; Derek Mendez; Sabine Botha; Steven Fried; Hao Hu; Reza Nazari; C. Swathi K. Menon; Joe Chen; Nipuna Weerasinghe; Sahba Zaare; Andre V. Struts; Suchithranga M. D. C. Perara; Roberto C. Alvarez; Shigeki Owada; Kensuke Tono; Petra Fromme; Richard A. Kirian; Michael F. Brown; Thomas D. Grant

doi:10.18957/rr.13.3.175

Section SACLA

Pump-probe TR-WAXS Distinguishes Activation from Heating Effects in Ultrafast Rhodopsin Dynamics

Konstantinos Karpos, Derek Mendez, Sabine Botha, Steven Fried, Hao Hu, Reza Nazari, C. Swathi K. Menon, Joe Chen, Nipuna Weerasinghe, Sahba Zaare, Andre V. Struts, Suchithranga M. D. C. Perara, Roberto C. Alvarez, Shigeki Owada, Kensuke Tono, Petra Fromme, Richard A. Kirian, Michael F. Brown, Thomas D. Grant

著者情報

Konstantinos Karpos
Arizona State University
Derek Mendez
SLAC National Accelerator Laboratory
Sabine Botha
Arizona State University
Steven Fried
University of Arizona
Hao Hu
Arizona State University
Reza Nazari
Arizona State University
C. Swathi K. Menon
University of Arizona
Joe Chen
University of Canterbury
Nipuna Weerasinghe
University of Arizona
Sahba Zaare
Arizona State University
Andre V. Struts
University of Arizona
Saint Petersburg State Medical University
Suchithranga M. D. C. Perara
University of Arizona
Roberto C. Alvarez
Arizona State University
Shigeki Owada
Japan Synchrotron Radiation Research Institute
Kensuke Tono
Japan Synchrotron Radiation Research Institute
Petra Fromme
Arizona State University
Richard A. Kirian
Arizona State University
Michael F. Brown
University of Arizona
Thomas D. Grant
SUNY University at Buffalo

キーワード: pump-probe, time-resolved WAXS, structural dynamics, visual rhodopsin, 2019B8039, BL3

ジャーナルオープンアクセス

2025 年 13 巻 3 号 p. 175-182

DOI https://doi.org/10.18957/rr.13.3.175

詳細

抄録

　TR-WAXS at SACLA BL3 examined structural dynamics in rhodopsin after retinal photoisomerization. We collected profiles up to 10ps post-green laser activation, using red light as control. SVD analysis showed one main signal per experiment. The rhodopsin response in green light differed from red light and from the CHAPS solvent control, suggesting distinct structural changes.

責任著者(Corresponding author)

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