A poorly differentiated squamous cell carcinoma cell line (SAS) from human tongue primary lesion was established.
In the cytoplasm of cultured cells, immunoperoxidase staining for human cytokeratin was strongly positive, and alkaline phosphatase staining revealed the enzyme reaction on the cell membranes.
Electron microscopic observations of SAS cells showed fully formed desmosomes and cytoplasmic filaments. In the 55th passage, population doubling time of culture cells was approximately 22 hours.
Chromosome analysis of cells showed hypertriploidy.
SAS cells were transplantable in nude mice, and the histological studies demonstrated preservation of the original histopathological features.
