悪性腫瘍細胞における微量元素定性分析に関する基礎的検討
1990 年 39 巻 4 号 p. 932-940
詳細
1990 年 39 巻 4 号 p. 932-940
It is important to study an essential metal metabolism of the cytoplasm and the microorganella in the cancer body. Various agents used for fixing and staining ultrathin sections for electron microscopic observation were evaluated their effects on x-ray microanalysis of essential metals in malignant tumor cells. The metal accumulation, especially platinum and copper in the cytoplasm was also investigated using ultrathin sections of materials treated by cisplatinum and copper sulfate. Electrolytes in VX2 carcinoma cells had been washed away during fixation or dehydration of the materials even after treatment with ammonium sulfate. However a peak of calcium was observed in the cytoplasm of specimens fixed by dithizone.
