A PRACTICAL ASSAY METHOD FOR DETERMINATION OF TETRACYCLINE AND OXYTETRACYCLINE IN TISSUES
1959 Volume 25 Issue 3 Pages 228-233
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1959 Volume 25 Issue 3 Pages 228-233
TOMIYAMA et al3) reported a practical assay method appropriate for the determination of minute amount of chlortetracycline (CTC) in tissues. The present study deals with the application of their method to the determination of tetracycline (TC) and oxytetracycline (OTC) in tissues.
It was found that the method could be applied for this purpose by only modifying the standard antibiotics solutions. The average recoveries of TC or OTC which was included in tissues were found 88% from muscle and 96% from skin. The minimum inhibitory concentration of TC or OTC was 0.006 mcg per cc., i.e., a detectable amount of OTC in tissues being 0.03 mcg per g. of tissue when one part of a sample tissue is extracted with four parts nation could be omitted by employing the linear regression line just as in the case of CTC.
The optimum condition for the sporulation of test organism, B. cereus # 213, has also been studied, since difficulty was frequently met in obtaining a high yield of spore of the test organism while none was experienced in case of B. cereus # 5. Several trials revealed that both a suitable cell concentration of the inoculum and a moderately thin layer of the basal medium are required for efficient sporulation of the organism.
