The proteinase in the pyloric caeca of bigeye tuna, Parathunnus sibi, was isolated according to the procedure described in Fig. 1 in a crystalline state (Fig. 2). Total activity of the crystalline enzyme corresponded to 2.7% of that of the original water extracts, and the specific activity of it was 21.1 times as high as that of the water extracts (Table 2).
Nippon Suisan Gakkaishi is an official journal of the Japanese Society of Fisheries Science written in Japanese only. The society publishes an English journal, Fisheries Science, as well, which accepts submissions from non-members of the society over the world. Detailed information is available in http://jsfs.jp/en/journals/fisheries-science.
