Biological Study on Ovulation in vitro of Fish-I
Effects of Pituitary and Chorionic Gonadotropins on Ovulation in vitro of Medaka, Oryzias latpes
1971 Volume 37 Issue 7 Pages 585-591
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1971 Volume 37 Issue 7 Pages 585-591
The effects of pituitary and chorinoic gonadotropins on ovulation in vitro of Oryzias latipes were studied.
The culture medium 199 containing calf serum was the most useful medium in this study. The eggs incubated in 199 containing the hormones were induced to ovulate between 1.00-4.00 a.m., and the ovulated eggs developed to the stage of blastoderm. Among the hormones used, LH of 4μg in concentration had the highest potency in inducing the ovulation in vitro. FSH 4μg, containing the trace of LH which could not induce the high percentage of ovulation, promoted ovulation. If there is no synergistic action at this level of both hormones, FSH may have the potency to induce ovulation. HCG as shown in the previous results, showed a considerably high percentage of ovulation.
