Purification of Fish Gastric Hyaluronidase

Abstract

Crude hyaluronidase, prepared by extraction with dilute acetic acid from gastric mucosa of mackerel, was purified 185 fold by fractionation with ammonium sulfate, filtration on Sephadex G-100 column and chromatographies on CM-cellulose and DEAE-cellulose columns. The final preparation was free from β-glucuronidase and β-N-acetylglucosami-nidase, and was homogeneous on disc electrophoresis conducted with polyacrylamide gel. It showed a high specific activity of 10, 000 turbidity reducing units of TOLKSDORF per milligram of its protein.