Studies on the Haptoglobin of Eel-I

Purification of Haptoglobin

Abstract

Haptoglobin (Hp), a hemoglobin (Hb)-binding protein, was isolated and purified from eel serum by a method which consists of Sephadex G-25 gel filtration, DEAF-cellulose batch adsorption, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration. The Hp specimen thus purified was homogeneous in starch gel electrophoresis at pH 8.4. The yield averaged about 20mg per 100ml of eel serum. It was noticed during the purification that, unlike rabbit Hp, prolonged standing in NaCI solution and adsorption to DEAE-cellulose results in a decrease of Hb-binding capacity.