Abstract
Several reports have been published on the cultivation conditions for Pasteurella piscicida, which are necessary for producing highly effective bacterins for the control of pseudotuberculosis in cultured yellowtail Seriola quinqueradiata. This report describes the effect of cultivation temperature, NaCl concentration and pH on the growth of two P. piscicida strains, 5866 and U-3, isolated from yellowtail. Todd Hewitt Broth was used for the examination of cultivation temperature, and a culture medium composed of polypeptone, yeast extract and NaCl was used for the examination of NaCl concentration and pH.
Growth of strains, 5866 and U-3, was observed at a temperature range of 15 to 32.5°C. The optimum temperature range was 22.5-30°C. NaCl at 0.5-5% was necessary for the growth of both strains. The optimum NaCl concentration for strain 5866 was 1-2% and for strain U-3 was 1-2.5%. Both strains grew in a pH range from 5.56 to 8.57. The optimum pH range was 6.47-7.24 for strain 5866 and 6.47-7.93 for strain U-3.
