Studies on Primary Cell Culture of Eel Leucocytes

Abstract

In vitro studies on culture of eel leucocytes were performed. Leucocytes were separated from peripheral blood and incubated in CO₂ incubator at 25°C. As for the morphological charac-teristics, some lymphocytes became larger and showed dark stained nucleus, but thrombocytes showed no changes. Neutrophils and monocytes spread out but they could not be distinguished from each other by the morphology. As for the changes during culture, some lymphocytes showed colony formation and the colonies became larger, that could show proliferation. Thrombocytes showed proliferation without colony formation. The spreading neutrophils and monocytes showed cluster and proliferation.
As results, it is appropriate to conclude that eel leucocytes can proliferate under culture con-ditions, and the way of proliferation is different for each type of leucocyte.