Anti-anemic Effect of Menadione Dimethylpyrimidinol Bisulfite against Molinate-induced Anemia in Common Carp

Abstract

Anti-anemic effect of menadione dimethylpyrimidinol bisulfite (MPB) against molinate-induced anemia was investigated in a bioassay test. Common carp fingerlings were arranged in 12 groups of 20 animals each. Five groups of fish were exposed to molinate at a concentration of 0.10 ppm, 6 groups of fish at 0.32 ppm, and one group of fish served as controls. Concentrations of MPB were set at 0.0, 0.4, 1.2, 3.6 and 10.8 ppb for the test solutions with 0.10 ppm of molinate, and at 0.0, 0.4, 1.2, 3.6, 10.8 and 32.4 ppb for those with 0.32 ppm of molinate, respectively. After 21-day exposure to molinate solutions with different concentrations of MPB, hemoglobin level was determined on all surviving fish. The mortality and hemoglobin level data indicated that MPB was highly effective in preventing anemia. The effective concentrations of MPB were estimated to to be 3.6 ppb in water containing 0.10 ppm of molinate and 32.4 ppb in water containing 0.32 ppm of molinate, respectively.