Abstract
The disinfectant effects of total residual oxidants (TROs) produced by ozonization of artificial seawater on fish pathogenic bacteria and viruses were investigated. A dose of0.1mg TROs/l was not stable in natural seawate, but was stable in artificial seawater with a bromine compound (sodium bromide). By the treatment with TROs of 0.1mg/l for 30 to 120 seconds, more than 99.9% of viable cells of Vibrio anguillarum, Aeromonas salmonicida, A. caviae, A. hydrophila, and Escherichia coli were killed, and 99.9% of infectivity of hirame rhabdovirus (HIRRV), infectious hematopoietic necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV), and yellowtail ascites virus (YAV) were inactivated. HIRRV was more than 99.7% inactivated by a treatment with a dose of 3.5g/m3 of ozone gas bubbles for 1 min.