抄録
This study aimed to investigate the direct effects of Triton WR-1339 on the production of fibrinogen in cells grown in vitro. HepG2 cells were incubated in Dulbecco's modified Eagle's medium (DMEM) for 6, 12 and 24 hr in the absence and presence of Triton WR-1339, used at four dose concentrations (0.12, 1.2, 12 and 120 μg/ml) . The high dose Triton WR-1339 treatment (120 μg/ml) for 24 hr induced significant cell toxicity as determined by the leakage of alanine aminotransferase (ALT) to the medium and by the cell viability using 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide (MTT) assay. Fibrinogen released into the medium from the HepG2 cells was measured by ELISA at 6, 12 and 24 hr. The low dose Triton WR-1339 treatments (0.12 and 1.2 μg/ ml) for 24 hr resulted in significantly increased fibrinogen secretion compared with the non-treated cells. Expression of α -, β - and γ -chains of fibrinogen at the mRNA level was determined by RT-PCR. After a 6 hr incubation with 0.12 and 12 μg / ml of Triton WR-1339, mRNA levels of γ -chain fibrinogen increased significantly. After 24 hr in the same treatment conditions, the fibrinogen β - and γ -chain mRNA levels were significantly increased, whereas the α - chain levels were unchanged as compared with non-treated controls. These results indicate that Triton WR-1339 induces the synthesis of fibrinogen by increasing the transcription of the β- and γ -chains of fibrinogen in HepG2 cells.
