1993 年 5 巻 1 号 p. 63-71
We analyzed the immunohistology and the quantitative temporal changes of immunotinctable endogenous vasodilators [calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal polypeptide (VIP) ] induced in the rat amygdaloid complex (corpus amygdaloideum) by one-hour cerebral Ischemia. Blood levels of these vascular agonists were also measured and compared with the Immunohistochemical observation results. The immunotinctability of CGRP immunoreactive fibers decreased significantly 12 h after cerebral Ischemia. CGRP concentration in the blood increased significantly in the 6 to 12 h after cerebral Ischemia, decreased slightly at 24 h, and returned to normal levels at 3 days. The immunotinctability of SP immunopositive fibers decreased significantly 12 h to 24 h after cerebral Ischemia, recovered slightly at 3 days, and returned to near normal level at 1 week. Blood levels of SP decreased significantly 12 h after cerebral Ischemia and appeared normal at 24 h and 3 days. No significant changes were observed in the blood levels of VIP nor in the immunotinctability of VIP immunopositive fibers from 3 hours to 2 weeks after cerebral Ischemia. Our results suggest that after cerebral Ischemia increases CGRP secretion into the blood stream and CGRP synthesis in the amygdaloid complex decreases. SP is synthesized in the amygdaloid complex and its release into the blood stream during the first 12 to 24 h and does not change the blood levels of VIP in this model. Hence the effects of cerebral Ischemia effects on individual vasodilators vary.
