Halophilic Starch-Binding Domain as a Novel Fusion Protein Partner for Efficient Recombinant Protein Expression

抄録

Halophiles produce halophilic enzymes which function well in high salinity conditions. The starch binding domain（SBD）of halophilic α-amylase has been found to show typical halophilic characteristics with high aqueous solubility and folding capacity. Here we developed a fusion protein expression vector, pSBF-S1, using this SBD as a fusion partner, looking for highly soluble protein expression and efficient affinity-purification. The fusion protein expressed could be purified by dual affinity chromatographies, i.e. Ni-affinity resin with N-terminal His-tag and amylose-resin with SBD affinity. The target protein is then generated through thrombin-digestion of fusion protein at the cleavage site between the target protein and SBD.