抄録
Our previous study demonstrated that the high-performance liquid chromatographic technique utilizing a plastic tube as a separation field allowed for analysis of amyloid fibrils. The method features the separation of analytes utilizing a polytetrafluoroethylene tube as a separation column, and detection of eluents using a fluorescence detector. In this study, we applied the method to the investigation of the effects of Al3+, Fe3+ and Zn2+ ions on the formation process of amyloid fibrils from hen egg white lysozyme. The increase in the number and intensity of peaks observed in the chromatograms suggests that amyloid fibrillation can be rapidly induced in the presence of metal ions.
