抄録
Ciguatera fish poisoning (CFP) is a form of food poisoning caused by the ingestion of a variety of reef fish, and it affects more than 50,000 people each year. The extremely low level of the causative neurotoxins, ciguatoxins, in fish has hampered the preparation of antibodies for detecting the toxins. In this paper, we have developed a thiol strategy to synthesize the keyhole limpet hemocyanin (KLH)-conjugate (11) of the ABCDE-ring fragment of Pacific ciguatoxins, CTX IB (1) and 54-deoxyCTX1B (4), and succeeded in production of a monoclonal antibody (3G8) against their left wings by immunizing mice with the hapten-KLH conjugate (11) as the synthetic antigen. The mAb 3G8 lacks the cross-reactivity with other related marine toxins. Sandwich enzyme-linked immunosorbent assay (ELISA) utilizing 3G8 and the previously prepared monoclonal antibody (8H4) enabled us to detect 1 specifically at lower than 0.5 ng/mL (nM).
