2020 Volume 32 Issue 187 Pages E99-E104
The recombinant production of glycoenzymes plays a significant role in industrial biocatalysis. The selection of an expression system which allows the high-level production of recombinant glycoenzymes using extracellular and intracellular expression, and the need for correct protein folding make Gram-positive bacteria interesting alternatives to the commonly used Gram-negative expression host Escherichia coli. In this overview we summarize recent approaches using Gram-positive bacteria as cell factories for the recombinant production of glycoenzymes, with a special focus on the optimization of glycoprotein production in lactic acid bacteria.