Substrate Specificity of Chondroitinase ABC-I Based on Analyses of Biochemical Reactions and Crystal Structure

Abstract

Chondroitinase ABC-I (cABC-I) has been studied for many years as an enzyme that degrades chondroitin sulfate (CS). There are several isomers of CS, and the reactivity with cABC-I is also different for each CS isomer. In this paper, we review the substrate specificity of cABC-I based on the results of analyzing cABC-I reactivity to CS that is more homogeneous, and the crystal structure of the cABC-I complex with unsaturated disaccharide derived from CS.