The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
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A Sandwich Enzyme-linked Immunosorbent Assay for Δ3, Δ2-Enoyl-CoA Isomerase in Rat-Tissue Homogenates
Hideaki Suzuki, Yoshihisa Tomioka, Shunji Ishiwata, Takahumi Yamaguchi, Koichi Miura, Takanori Hishinuma, Michinao Mizugaki
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Keywords: sandwich ELISA, Δ3, Δ2-enoyl-CoA-isomerase, monoclonal antibody, β-oxidation of unsaturated fatty acids
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1996 Volume 179 Issue 1 Pages 35-45

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Abstract
We established two monoclonal antibodies (MAbs, A6-1-E4, 1gM; κ and BC-D1, IgG1, λ), that specifically recognize different epitopes of rat Δ3, Δ2-enoyl-CoA isomerase (ECI). We then developed a specific and reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using a capture monoclonal antibody and an indicator polyclonal antibody to evaluate ECI. The amounts of ECI were linearly determined in the ranges from 15 to 250 ng/well by this method. This assay can detect the amount of ECI in tissue homogenates without subfractionation. The ECI levels were 0.13 ng/μg homogenate in normal rat liver and 1.50 ng/μg homogenate in clofibrate-treated rat liver.
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© 1996 Tohoku University Medical Press
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