The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
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A Sandwich Enzyme-linked Immunosorbent Assay for Δ3, Δ2-Enoyl-CoA Isomerase in Rat-Tissue Homogenates
Hideaki Suzuki, Yoshihisa Tomioka, Shunji Ishiwata, Takahumi Yamaguchi, Koichi Miura, Takanori Hishinuma, Michinao Mizugaki
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キーワード: sandwich ELISA, Δ3, Δ2-enoyl-CoA-isomerase, monoclonal antibody, β-oxidation of unsaturated fatty acids
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1996 年 179 巻 1 号 p. 35-45

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We established two monoclonal antibodies (MAbs, A6-1-E4, 1gM; κ and BC-D1, IgG1, λ), that specifically recognize different epitopes of rat Δ3, Δ2-enoyl-CoA isomerase (ECI). We then developed a specific and reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using a capture monoclonal antibody and an indicator polyclonal antibody to evaluate ECI. The amounts of ECI were linearly determined in the ranges from 15 to 250 ng/well by this method. This assay can detect the amount of ECI in tissue homogenates without subfractionation. The ECI levels were 0.13 ng/μg homogenate in normal rat liver and 1.50 ng/μg homogenate in clofibrate-treated rat liver.
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© 1996 Tohoku University Medical Press
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    The Tohoku Journal of Experimental Medicine (TJEM) was founded in 1920 by professors of Tohoku Imperial University, Medical School. The TJEM has been published continuously, except for the year of 1946 just after the World War II. The TJEM is open to original articles in all branches of medical sciences. The TJEM also covers the fields of disaster-prevention science, including earthquake archeology.


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