Rhodostomin Inhibits the Transforming Growth Factor-β1-Enhanced Adhesion Activity of ROS 17/2.8 Osteosarcoma Cells

Abstract

We have investigated the effect of transforming growth factor-β1 (TGF-β1) on the in vitro adhesion activity of the rat osteosarcoma cell lines (ROS 17/2.8) to extracellular matrix substrata, including fibronectin, type I and IV collagen, as well as laminin. The interaction of Arg-Gly-Asp (RGD) and rhodostomin, an RGD containing snake venom, with TGF-β1 on the cell adhesion was also evaluated. The results showed that incubation with various concentration of TGF-β1 (1-15 ng/ml) significantly increased the adhesion activity (1.4 to 2.5 folds) of ROS 17/2.8 to fibronectin and type I collagen (p<0.01), whereas the adhesion activity to laminin and type IV collagen was slightly elevated (1.1 to 1.5 folds). The peak effect of TGF-β1 on the cell adhesion occurred after pretreatment of ROS 17/2.8 with TGF-β1 for 6 hours. Treatment with Arg-Gly-Asp-Ser (RGDS) and rhodostomin effectively suppressed the TGF-β1-enhanced adhesion activity to fibronectin and type I collagen. This study demonstrated that the up-regulated cell adhesion activity of ROS 17/2.8 cells by the TGF-β1 can be inhibited by the rhodostomin.