Measurement of Phosphatidylcholine Hydroperoxide in Mild Ischemia-Reperfusion Injury in Rat Intestine

TSUYOSHI MASUKO; YUJI FUNAYAMA; HIROO NAITO; KOHEI FUKUSHIMA; CHIKASHI SHIBATA; KEN-ICHI TAKAHASHI; HITOSHI OGAWA; TATSUYA UENO; AKIHIKO HASHIMOTO; TERUO MIYAZAWA; KIYOTAKA NAKAGAWA; SEIKI MATSUNO; IWAO SASAKI

doi:10.1620/tjem.198.223

Regular Contributions

Measurement of Phosphatidylcholine Hydroperoxide in Mild Ischemia-Reperfusion Injury in Rat Intestine

TSUYOSHI MASUKO, YUJI FUNAYAMA, HIROO NAITO, KOHEI FUKUSHIMA, CHIKASHI SHIBATA, KEN-ICHI TAKAHASHI, HITOSHI OGAWA, TATSUYA UENO, AKIHIKO HASHIMOTO, TERUO MIYAZAWA, KIYOTAKA NAKAGAWA, SEIKI MATSUNO, IWAO SASAKI

Author information

TSUYOSHI MASUKO
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
YUJI FUNAYAMA
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
HIROO NAITO
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
KOHEI FUKUSHIMA
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
CHIKASHI SHIBATA
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
KEN-ICHI TAKAHASHI
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
HITOSHI OGAWA
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
TATSUYA UENO
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
AKIHIKO HASHIMOTO
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
TERUO MIYAZAWA
Depertment of Applied Biochemistry, Tohoku University, School of Agriculture
KIYOTAKA NAKAGAWA
Depertment of Applied Biochemistry, Tohoku University, School of Agriculture
SEIKI MATSUNO
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine
IWAO SASAKI
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine

Keywords: lipid peroxide, intestine, ischemia-reperfusion injury, rat

JOURNAL FREE ACCESS

2002 Volume 198 Issue 4 Pages 223-232

DOI https://doi.org/10.1620/tjem.198.223

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Abstract

In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.

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