A Comparative Study of the Colorimetric Method of Folin, Cannon and Denis and that of Suto and Inouye for the Determination of Epinephrine. (Experiments on the Medulla of the Bovine Suprarenal Gland.)

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By the application of liquid air for pulverizing and mixing the medullary tissue (cortex or whole gland) of the suprarenal body, we obtained the “identically uniform” material for comparing different methods of extraction and estimation for epinephrine, as those of Folin and Suto, with each other.
The values of epinephrine in the suprarenal medulla of cow and ox, obtained by Folin, Cannon and Denis and by Suto and Inouye, coincide well with each other, especially in the averages, though there were noted some dissimilarities of a small degree in some individual cases. The values yielded by extracting by either Folin or Suto and estimating by the rabbit intestine segment method showed quite the same tendency.
It may be therefore concluded that both methods, Folin, Cannon and Denis, and Suto and Inouye, have the same valuation for extracting and estimating epinephrine in the bovine suprarenal medulla.
As to the ratio of the epinephrine content of the extract prepared by either Folin or Suto, determined colorimetrically by either Folin or Suto respectively to that estimated by the rabbit intestine strip method the present experiments yielded the same values as those given in our previous paper.²⁾ Namely, the ratio of the colorimetrical value to the intestine value was noted as 1:2 in the averages for both the colorimetrical determinations taken altogether.