Abstract
The incorporation rates of radioactivity into both lipid and acid soluble fractions of mouse tissues such as liver, kidney and small intestine and others were followed up at various time intervals (5min -24hr) after the intraperitoneal administration of ethanolamine-2-14C. The percentages of radioactivity of individual phospholipids were also determined in three tissues. The quantitative isolation of individual radioactive compounds from acid soluble fractions was performed using various chromatographic techniques.
The pool sizes and the specific activities of ethanolamine and its metabolites such as phosphoryl-ethanolamine, glycerylphosphoryl-ethanolamine, phosphatidylethanolamine, lysophosphatidyl-ethanolamine and phosphatidyl-choline were determined at various time intervals. From their values the turnover rates of various reactions in phosphatidyl-ethanolamine metabolism in mouse liver, kidney and small intestine were estimated by means of Zilversmit's equation.
