Studies on Radioactive Iodination of Fluorescein Isothiocyanate

Abstract

The present paper deals with radioactive iodination of fluorescein isothiocyanate (FITC) in chloroform. In the procedure, FITC in phosphate buffer at pH 7.0 was added to radioactive iodine at molecular ratio 1:1.5 or more. Some derivatives containing various numbers of iodine were prepared at the reaction in accordance with the molecular ratio. Starch-gel electrophoresis, thin layer chromatography, spectrophotometric analysis, infrared spectra, elementary analysis and nuclear magnetic resonance of the products showed that several derivatives incorporated with various numbers of iodine were synthesized and that isothiocyanate group of most of the derivatives was not separated at the reaction.