The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
The Kinetics of Glycerol and Dimethyl Sulfoxide in Cultured Leukemic and Burkitt Lymphoma Cells
GEN NIWAYAMA
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キーワード: dimethyl sulfoxide, glycerol, cryoprotective agents, cell preservation
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1972 年 106 巻 1 号 p. 17-30

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This experiment was designed to study the kinetics of glycerol and dimethyl sulfoxide (DMSO) in cultured cells of human leukemia and lymphoma at 0°C and 37°C. Quantitative photographic measurement of changes of cell diameter was used as assay of relative permeability. Movement of DMSO was generally found to be more rapid than that of glycerol. Movement of both agents was found to be more rapid at 37°C than at 0°C. Changes in cell diameter was marked with glycerol than with DMSO. Equilibration time of DMSO, as assayed by change in cell diameter at 0°C was approximately 15 to 20 minutes as contrasted with 20-25 minutes for glycerol.

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    The Tohoku Journal of Experimental Medicine (TJEM) was founded in 1920 by professors of Tohoku Imperial University, Medical School. The TJEM has been published continuously, except for the year of 1946 just after the World War II. The TJEM is open to original articles in all branches of medical sciences. The TJEM also covers the fields of disaster-prevention science, including earthquake archeology.


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