Abstract
Qualitative and quantitative determination of urinary acid mucopolysaccharide has been studied by several methods including ECTEOLA-cellulose column chromatography, Dowex 1×2 column chromatography, electrophoresis, and enzymatic digestion with testicular hyaluronidase and chondroitinase. To avoid the possibility of loss of urinary acid mucopolysaccharides, especially of keratosulfate, cetylpyridinium chloride as an acid mucopolysaccharide precipitant and dialytic procedure were not used. 1) The normal male children excreted 8.0mg of uronic acid and 8.5mg of hexosamine/ liter of urine, and the uronic acid (mg)/creatinine (g) ratio was 12.8 and the hexosamine (mg)/creatinine (g) ratio was 13.8. 2) The urinary acid mucopolysaccharides in the normal male children comprised mostly chondroitin sulfate A (47.6%) and chondroitin sulfate C (34.2%). 3) Non-sulfated acid, mucopolysaccharides comprised 10.4% of the total acid mucopolysaccharide excreted, consisted of hyaluronic acid (3.3%) and chondroitin (7.1%) respectively. 4) The remaining acid mucopolysaccharides consisted of heparitin sulfate (3.4%), chondroitin sulfate B (2.8%) and keratosulfate (1.6%).
