抄録
An effort has been made on dogs to determine whether adenosine-3', 5'-monop'iosphate (3', 5'-AMP) and 5-hydroxytryptamine exert a direct effect on the 17-oxosteroid secretion by the testis, and to clarify whether the secretory response to 3', 5'-AMP is affected or not by the prior treatment with xnethylenedianiline, an inhi-bitor of steroidogenesis. 3', 5'-AMP (10 mg/kg) injected into the testicular tissue caused considerably increased secretion of 17-oxosteroids by the testis, although an intravenous injection of 3', 5'-AMP (20 mg/kg) was without effect, since it did not significantly alter the secretion of testicular 17-oxosteroids. The intravenous administration of 0.5 mg/kg of 5-hydroxytryptamine or intratesticular injection of total dose of 1.0 mg failed to produce a notable increase in the secretion of 17-oxosteroids in any instance. In dogs pre-treated with methylenedianiline (20 mg/kg), the 17-oxosteroid secretory response of the testis to an intratesticular injection of 3', 5'-AMP was completely abolished: no 17-oxosteroids were secreted from the testis. It is thus suggested that 3', 5'-AMP has the gonadotrophicc effect and its effect can be suppressed by pre-treatment with methylenedianiline, and that 5-hydroxytryptamine has no direct stimulatory effect on 17-oxosteroid secretion.
