The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Increase of Dermatan Sulfate in a Case of Pulmonary Fibrosis
MASAKICHI MOTOMIYA, HIDEO ARAI, HIROSI SATO, ATSUNOBU YOKOSAWA, HIROMI NAGAI, KIYOSHI KONNO
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Keywords: dermatan sulfate, pulmonary fibrosis
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1975 Volume 115 Issue 4 Pages 361-365

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Abstract
The quantity and composition of acid glycosaminoglycans (mucopolysaccharides) were compared between a fibrotic lung and normal lungs. The acid glycosaminoglycans were isolated by proteolysis, fractionation with ethanol and precipitation with cetylpyridinium chloride, and were identified by chromatography, electrophoresis and incubation with mucopoly-saccharide-lyases. Evidence was obtained for the presence of hyaluronic acid, chondroitin sulfate A(C), dermatan sulfate and heparan sulfate. Quantitation of individual glycosaminoglycans by using specific mucopolysaccharide-lyases revealed that the quantity of dermatan sulfate in the fibrotic lung exceeded that in the normal lungs.
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