The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Antigenicity of Methylated Deoxyribonucleic Acid
JUNKO IGARASHI, EIJI OKUHARA
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キーワード: anti-Me-DNA antibody, immunoglobulin
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1976 年 120 巻 1 号 p. 25-30

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Methylated salmon sperm deoxyrinbonucleic acid (Me-ssDNA) prepared by using dimethyl sulfate was used to elicit antibodies in rabbits. The antisera obtained reacted specifically with Me-ssDNA but not with thermally denatured Me-DNA, native DNA, thermally denatured DNA or ribonucleic acids (RNAs). Antibody activity was demonstrable in both IgG and IgM fractions which were obtained by Sephadex G-200 column chromatography. A single precipitating line was formed by either of IgG and IgM fractions in the double diffusion test with Me-ssDNA.
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    The Tohoku Journal of Experimental Medicine (TJEM) was founded in 1920 by professors of Tohoku Imperial University, Medical School. The TJEM has been published continuously, except for the year of 1946 just after the World War II. The TJEM is open to original articles in all branches of medical sciences. The TJEM also covers the fields of disaster-prevention science, including earthquake archeology.


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