The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
A Simple Method of Cell-Mediated Cytotoxic Assay by Postlabeling of Tritiated Thymidine
KENTARO TOHKOKICHI KIKUCHI
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1977 Volume 122 Issue 3 Pages 259-265

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Abstract

The cytotoxic reaction of effector cells on target cells was performed on cover slips which were set in a petri dish so as to be interposed from the dish surface through the bases of the slide glasses. After incubation, the target cells remaining on the cover slips were pulse-labeled with 3H-TdR for 45 min. Subsequently, the cover slips were removed from the bases, and dipped in cold 5% TCA to be freed from non-incorporated isotopes. The residual target cells adhering on the cover slips were counted without being removed. By this assay system, the specific or non-specific cytotoxicity of lymphocytes or macrophages was easily demonstrated with reliability.

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