Differentiative and Proliferative Effects of (But)₂cAMP, n-Butyric Acid and Prednisolone on the Malignant Melanoma Cell Line (TM-1) in Vitro and in Vivo

抄録

Both (But)₂cAMP and n-butyric acid suppressed the proliferation of TM-1 cells and induced the formation and elongation of the cellular processes in vitro. Prednisolone stimulated the proliferation of TM-1 cells and increased the number of polygonal cells in vitro. These results indicated that (But)₂cAMP and n-butyric acid induce the differentiation of TM-1 cells, whereas prednisolone brings the cells in an undifferentiated state. The growth of TM-1 cell tumor transplanted into nude mice was suppressed by 5mg of (But)₂cAMP and I mg of theophylline. Remarkable histological changes were seen in the tumors treated with these agents as compared with untreated tumors. This suggests that (But)₂cAMP suppresses the proliferation of TM-1 cells in vivo.