抄録
A method was established to isolate the glomeruli from the kidney fixed in formaldehyde. O-Hydroxylysylglycosides and hydroxyproline in the isolated glomeruli from the kidney of normal subjects, diabetics and subjects with chronic glomerulonephritis were measured on the alkaline hydrolyzate of the glomeruli. O-Hydroxylysylglycosides in 1000 glomeruli were 9.42±2.68 nmoles (mean±s. D. ) for controls, 12.92±7.36 nmoles for diabetics and 12.54±4.62 nmoles for subjects with chronic glomerulonephritis. Hydroxyproline in 1000 glomeruli from control, diabetics and subjects with chronic glomerulonephritis was 43.0±10.7, 82.0±39.3 and 52.1±21.3 nmoles, respectively. Hydroxyproline in the hyalinized glomeruli from diabetics was 123.3±22.4 nmoles/1000 glomeruli, and significantly increased in comparison with that in the glomeruli from chronic glomerulonephritis or control subjects.
